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Hand hygiene01:23

Hand hygiene

Asepsis is the practice of preventing or breaking the chain of infection. The nurse employs aseptic techniques to prevent the spread of microorganisms and reduce the risk of diseases. Hand hygiene is the cornerstone of aseptic techniques and is classified into medical and surgical asepsis. Medical asepsis includes hand hygiene and the use of gloves. Surgical asepsis, or the sterile technique, refers to practices that render and keep objects and areas free of microorganisms.
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Preferred use of polyhexanide in orthopedic surgery.

Eric Röhner1, Jörn B Seeger, Paula Hoff

  • 1Department of Traumatology and Orthopedics, Charité – Universitätsmedizin, Berlin, Germany. eric.roehner@charite.de

Orthopedics
|September 30, 2011
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Polyhexanide, a common antiseptic in orthopedic surgery, was found to induce apoptosis in human chondrocytes. This finding suggests caution when using polyhexanide in septic joint procedures.

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Area of Science:

  • Biomedical research
  • Cell biology
  • Orthopedic surgery

Background:

  • Polyhexanide is widely used in orthopedic surgery due to its antimicrobial properties and tissue compatibility.
  • Recent research indicates potential toxic effects of polyhexanide on human chondrocytes.

Purpose of the Study:

  • To investigate the toxic and apoptotic effects of polyhexanide on primary human chondrocytes.
  • To determine if polyhexanide induces apoptosis in human chondrocytes.

Main Methods:

  • Primary human chondrocytes were isolated from patients undergoing knee arthroplasty.
  • Cells were exposed to 0.04% polyhexanide.
  • Apoptosis was analyzed using flow cytometry (Annexin V, active caspases, 7AAD) and fluorescence microscopy (Annexin V, propidium iodide).

Main Results:

  • Polyhexanide significantly increased Annexin V and active caspases expression in chondrocytes.
  • Fluorescence microscopy revealed a high proportion of early apoptotic cells (Annexin V positive, propidium iodide negative).

Conclusions:

  • Polyhexanide promotes apoptosis in primary human chondrocytes in vitro.
  • These findings may have implications for the use of polyhexanide in septic joint surgery.