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Related Experiment Video

Updated: May 29, 2026

A Quantitative Glycomics and Proteomics Combined Purification Strategy
11:38

A Quantitative Glycomics and Proteomics Combined Purification Strategy

Published on: March 8, 2016

High throughput plasma N-glycome profiling using multiplexed labelling and UPLC with fluorescence detection.

Ana Knežević1, Jonathan Bones, Stjepan Krešimir Kračun

  • 1Genos Ltd, Laboratory for Glycobiology, Zagreb, Croatia.

The Analyst
|September 30, 2011
PubMed
Summary

This study introduces a fast glycomic profiling method for plasma N-glycans. The technique significantly increases sample throughput, enabling analysis of hundreds of samples daily.

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Area of Science:

  • Glycomics
  • Analytical Chemistry
  • Biochemistry

Background:

  • Glycomic profiling is crucial for understanding biological processes.
  • Current methods can be time-consuming, limiting large-scale studies.
  • High-throughput analysis is needed for clinical and research applications.

Purpose of the Study:

  • To develop a rapid and multiplexed method for N-glycan profiling.
  • To enhance sample throughput for plasma glycomic analysis.
  • To enable efficient large-scale glycomic studies.

Main Methods:

  • N-glycans from plasma samples were individually labeled with specific fluorescent tags (aniline, 2-aminobenzamide, 2-aminoacridone).
  • Labeled N-glycans were mixed and co-injected for separation using Hydrophilic Interaction Liquid Chromatography (HILIC) with fluorescence detection.

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Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
13:21

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Published on: May 4, 2012

Related Experiment Videos

Last Updated: May 29, 2026

A Quantitative Glycomics and Proteomics Combined Purification Strategy
11:38

A Quantitative Glycomics and Proteomics Combined Purification Strategy

Published on: March 8, 2016

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
13:21

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Published on: May 4, 2012

  • The multiplexed method was transferred to Ultra-Performance Liquid Chromatography (UPLC) for increased speed and throughput.

    • Main Results:

    • A single HILIC-fluorescence run allowed simultaneous separation of multiplexed N-glycans.
    • Transfer to UPLC-fluorescence increased sample throughput from 24 to 864 plasma samples per day.
    • The method provides rapid and efficient glycomic profiling.

    Conclusions:

    • The developed method offers a significant advancement in the speed and efficiency of plasma N-glycan analysis.
    • This high-throughput glycomic profiling technique can facilitate larger clinical and research studies.
    • The multiplexed approach enhances analytical capacity for glycomic investigations.