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Updated: May 29, 2026
Creation of Colonic Anastomosis in Mice
Published on: January 17, 2019
The effects of iloprost on colonic anastomotic healing in rats
G Galanopoulos1, M G Pramateftakis, D Raptis
14th Surgical Department, Aristotle University of Thessaloniki, 2 Macedonias Str, PO 55535, Pilea, Thessaloniki, Greece.
Purpose:
The purpose of this experimental study is to investigate the effects of iloprost on colonic anastomotic healing in rats, after intraperitoneal administration.
Methods:
Forty male Albino-Wistar rats were randomized into two groups of twenty animals each. They all underwent colonic resection followed by an inverted anastomosis. The rats of Group A (control) received 3 ml of NaCl intraperitoneally, while those of Group B (iloprost) received iloprost (2 μg/kg body weight), immediately postoperatively and daily until killed. Each group was further divided into two equal subgroups, depending on the day of killing. The animals of subgroups 1 were killed on the fourth postoperative day, while those of subgroups 2 on the eighth. Macroscopical and histological assessments were performed. Besides, anastomotic bursting pressures and the tissue concentrations in hydroxyproline and collagenase I were also evaluated.
Results:
No anastomotic dehiscence was noted. The mean bursting pressure was higher in the iloprost group compared with the control group, but a significant difference was revealed only on the fourth postoperative day. Furthermore, iloprost significantly increased the new vessel formation on the fourth, as well as on the eighth postoperative day.
Conclusion:
Iloprost enhances the early phase of colonic anastomotic healing in rats.
