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Updated: May 29, 2026

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center
Published on: September 28, 2022
Engineering cyclic amidases for non-natural amino acid synthesis
Francisco Javier Las Heras-Vázquez1, Josefa María Clemente-Jiménez, Sergio Martínez-Rodríguez
1Departamento de Química-Física, Bioquímica y Química Inorgánica, Universidad de Almería, Almería, Spain. fjheras@ual.es
Abstract:
Hydantoinases/dihydropyrimidinases are important biotechnological enzymes involved in the production of α- and β-amino acids. Their isolation from new sources with different substrate specificities, improved activity, enantioselectivity, or higher stability continues to be of great industrial interest. Here, we provide a detailed description of how to produce high quantities of the recombinant hydantoinase/dihydropyrimidinase enzyme from Sinorhizobium meliloti CECT4114 (SmeDhp). Several techniques are combined to obtain this goal, from cloning to activity measurement by HPLC.
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