Site-specific incorporation of unnatural amino acids as probes for protein conformational changes
Jennifer C Peeler1, Ryan A Mehl
1Department of Chemistry, Franklin & Marshall College, Lancaster, PA, USA.
Abstract:
Site-specific in vivo incorporation of unnatural amino acids provides powerful tools for the study of protein interaction and dynamics. Here, we provide a protocol for the incorporation of six such UAA probes into a GFP reporter system, expressed in Escherichia coli from both arabinose and lactose-inducible expression plasmids using an autoinduction media.
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