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Updated: May 29, 2026

Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins
Published on: October 4, 2017
Site-specific modification of proteins by the staudinger-phosphite reaction
Paul Majkut1, Verena Böhrsch, Remigiusz Serwa
1Institut für Chemie und Biochemie, Freie Universität Berlin, Berlin, Germany.
Abstract:
Chemoselective reactions are important tools for the modification of peptides and proteins. Thereby the modification is desired to be site specific and bioorthogonal. Here we describe the site-specific modification of azido-proteins via a Staudinger-type phosphite ligation. The reaction was carried out in aqueous system on proteins containing p-azido-phenylalanine in a single position introduced by the amber codon technique. A selective introduction of branched polyethylene scaffolds can be achieved with the application of the methodology reported herein.
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