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Assay of amino acid racemases

Masumi Katane1, Masae Sekine, Hiroshi Homma

  • 1Department of Pharmaceutical Life Sciences, Kitasato University, Minatoku, Tokyo, Japan.

Methods in Molecular Biology (Clifton, N.J.)
|September 30, 2011
PubMed

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A racemic mixture, or racemate, is an equimolar mixture of enantiomers of a molecule that can be separated using their unique interaction with chiral molecules or media. Racemic mixtures are denoted by the (±)- prefix. This ‘optical rotation descriptor’ applies to the whole solution of a racemic mixture rather than a specific stereoisomer. Enantiomers typically have the same physical and chemical properties. Hence, they are not easily separable. However, enantiomers can exhibit different...