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Rup (RNA-seq Usability Assessment Pipeline) - Quality Control for Bulk RNA-seq Experiments in Eukaryotes
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Nuclear quality control of RNA polymerase II transcripts
Manfred Schmid1, Torben Heick Jensen
1Centre for mRNP Biogenesis and Metabolism, Department of Molecular Biology, Aarhus University, C.F. Møllers Alle, Bldg. 130, 8000 Aarhus C., Denmark.
Wiley Interdisciplinary Reviews. RNA
|September 30, 2011
Summary
Cells possess nuclear RNA quality control mechanisms to degrade or silence aberrant RNA transcripts, preventing interference with normal cellular processes and gene regulation.
Area of Science:
- Molecular Biology
- Cell Biology
- Genetics
Background:
- Eukaryotic RNA polymerase II generates diverse RNA transcripts requiring maturation.
- Aberrant RNA accumulation can disrupt cellular RNA metabolism and pose risks.
- RNA maturation involves capping, splicing, polyadenylation, and protein packaging.
Purpose of the Study:
- To review the mechanisms of nuclear RNA quality control in eukaryotic cells.
- To highlight the importance of degrading or silencing unwanted RNA species.
- To connect RNA quality control pathways to gene regulation.
Main Methods:
- Literature review of RNA processing and quality control pathways.
- Analysis of mechanisms for aberrant RNA degradation, retention, and silencing.
- Examination of the role of RNA quality control in normal gene expression.
Main Results:
- Cells employ sophisticated nuclear RNA quality control systems.
- These systems target aberrant transcripts, including transcriptional noise.
- Mechanisms for mRNA quality control are well-characterized and inform other pathways.
- RNA quality control is integral to preventing cellular damage and regulating gene expression.
Conclusions:
- Nuclear RNA quality control is essential for maintaining cellular homeostasis.
- These pathways prevent the accumulation of potentially harmful RNA species.
- RNA quality control mechanisms are linked to broader gene regulatory networks.
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