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Related Experiment Video

Updated: May 29, 2026

Reconstitution of a Kv Channel into Lipid Membranes for Structural and Functional Studies
10:22

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Published on: July 13, 2013

AMP-activated protein kinase downregulates Kv7.1 cell surface expression.

Martin N Andersen1, Katarzyna Krzystanek, Thomas Jespersen

  • 1The Danish National Research Foundation Centre for Cardiac Arrhythmia, Department of Biomedical Sciences, The Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3, Copenhagen, Denmark.

Traffic (Copenhagen, Denmark)
|October 1, 2011
PubMed
Summary

The potassium channel Kv7.1 is internalized and degraded in lysosomes during cell polarization, mediated by AMP-activated protein kinase (AMPK) and the E3 ubiquitin ligase Nedd4-2.

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11:02

Exploring Arterial Smooth Muscle Kv7 Potassium Channel Function using Patch Clamp Electrophysiology and Pressure Myography

Published on: September 14, 2012

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Physiology

Background:

  • Potassium channel Kv7.1 is crucial for cardiac repolarization and epithelial salt/water transport.
  • Cell polarization triggers Kv7.1 internalization via protein kinase C (PKC).

Purpose of the Study:

  • To elucidate the downstream pathway of PKC leading to Kv7.1 internalization during cell polarization.
  • To identify the molecular mechanisms regulating Kv7.1 trafficking in polarized epithelial cells.

Main Methods:

  • Confocal microscopy to track Kv7.1 endocytosis.
  • Pharmacological activation of AMP-activated protein kinase (AMPK).
  • Xenopus oocyte electrophysiology to assess Kv7.1 currents.

Main Results:

  • Kv7.1 is endocytosed and degraded via the lysosomal pathway upon MDCK cell polarization.
  • AMPK activation mimics Kv7.1 internalization.
  • AMPK activates the E3 ubiquitin ligase Nedd4-2, which mediates Kv7.1 downregulation.
  • Nedd4-2-dependent mechanism controls Kv7.1 currents in Xenopus oocytes.

Conclusions:

  • Kv7.1 internalization during epithelial cell polarization is an AMPK- and Nedd4-2-dependent process.
  • This pathway targets surface-expressed Kv7.1 channels for lysosomal degradation.