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Related Experiment Video

Updated: May 29, 2026

Use of an Influenza Antigen Microarray to Measure the Breadth of Serum Antibodies Across Virus Subtypes
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Use of an Influenza Antigen Microarray to Measure the Breadth of Serum Antibodies Across Virus Subtypes

Published on: July 26, 2019

Cytometric microsphere array for subtyping avian influenza virus.

Fan Zhang1, Mingqiang Zou, Jinfeng Li

  • 1Chinese Academy of Inspection and Quarantine, Beijing, China.

Viral Immunology
|October 1, 2011
PubMed
Summary
This summary is machine-generated.

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A new microsphere assay enables rapid subtyping of avian influenza virus (AIV). This method efficiently detects specific AIV strains like H5N1 and H9N2, aiding in disease control.

Area of Science:

  • Veterinary Virology
  • Molecular Diagnostics
  • Biotechnology

Background:

  • Avian influenza virus (AIV) poses a significant threat to poultry health and global food security.
  • Accurate and rapid subtyping of AIV strains is crucial for effective disease surveillance and control strategies.
  • Existing methods for AIV subtyping can be time-consuming and labor-intensive.

Purpose of the Study:

  • To develop and validate a novel microsphere-based fluorescent assay for the simultaneous subtyping of avian influenza virus (AIV).
  • To assess the sensitivity and specificity of the developed assay for detecting common AIV subtypes, including H5N1 and H9N2.
  • To establish a powerful tool for multiplex pathogen detection in veterinary diagnostics.

Main Methods:

  • Development of a microsphere-based assay utilizing encoded beads covalently bound with AIV subtype-specific DNA probes.

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Last Updated: May 29, 2026

Use of an Influenza Antigen Microarray to Measure the Breadth of Serum Antibodies Across Virus Subtypes
08:52

Use of an Influenza Antigen Microarray to Measure the Breadth of Serum Antibodies Across Virus Subtypes

Published on: July 26, 2019

A Miniaturized Glycan Microarray Assay for Assessing Avidity and Specificity of Influenza A Virus Hemagglutinins
10:32

A Miniaturized Glycan Microarray Assay for Assessing Avidity and Specificity of Influenza A Virus Hemagglutinins

Published on: May 29, 2016

High-throughput Detection Method for Influenza Virus
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High-throughput Detection Method for Influenza Virus

Published on: February 4, 2012

  • Amplification of target DNA fragments using biotinylated primers.
  • Hybridization of denatured DNA with probes on encoded beads, followed by detection with quantum dots-streptavidin (QDs-streptavidin).
  • Screening of reaction products using flow cytometry for simultaneous identification and subtyping of AIV strains.

    • Main Results:

    • The assay successfully determined and subtyped AIV strains, distinguishing between subtypes like H5N1 and H9N2 based on bead and QD fluorescence.
    • High sensitivity was achieved, with detection thresholds of 74 pg for H5N1 and 1 pg for H9N2 nucleic acids.
    • The method demonstrated potential for simultaneous detection of multiple pathogens or multiple types of a single pathogen.

    Conclusions:

    • The established microsphere-based fluorescent assay is a sensitive and specific tool for avian influenza virus subtyping.
    • This assay offers a powerful platform for rapid, multiplexed detection of veterinary pathogens.
    • The findings support the application of this technology for enhanced AIV surveillance and diagnostics.