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Cell analysis using a multiple internal reflection photonic lab-on-a-chip.

Jordi Vila-Planas¹, Elisabet Fernández-Rosas, Bergoi Ibarlucea

¹Centre Nacional de Microelectrònica (IMB-CNM, CSIC), Barcelona, Spain.

Nature Protocols

|October 1, 2011

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We developed a photonic lab-on-a-chip (PhLoC) for rapid cell analysis. This low-cost device enables quick cell counting and viability assessment, making it suitable for point-of-care applications.

Area of Science:

Biophotonics
Microfluidics
Cell Biology

Background:

Accurate cell analysis is crucial for diagnostics and research.
Existing methods can be time-consuming and expensive.
Need for rapid, cost-effective cell analysis tools.

Purpose of the Study:

To present a protocol for cell culture analysis using a photonic lab-on-a-chip (PhLoC).
To demonstrate the PhLoC's capability for rapid cell counting and viability estimation.
To highlight the PhLoC's potential for point-of-care applications.

Main Methods:

Utilized a photonic lab-on-a-chip (PhLoC) with a broadband light source and spectrometer.
Operated the PhLoC in three regimes: light scattering, light scattering plus absorption, and absorption.

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Fabricated the PhLoC using a single-mask soft lithography process with low-cost materials.

Main Results:

Achieved an acquisition time of approximately 30 ms for cell spectrum analysis.
Demonstrated rapid cell counting, dead/live ratio estimation, and multiparametric measurements.
The entire protocol, from substrate to results, can be completed in 29 hours.

Conclusions:

The PhLoC offers a rapid, cost-effective, and versatile platform for cell analysis.
Its simple fabrication and low cost facilitate implementation in point-of-care settings.
The developed protocol enables efficient multiparametric cell characterization.