Related Experiment Video
Updated: May 29, 2026
Autonomously Bioluminescent Mammalian Cells for Continuous and Real-time Monitoring of Cytotoxicity
Published on: October 28, 2013
Non-invasive monitoring of cytotoxicity based on kinetic changes of cellular autofluorescence
Artur Bednarkiewicz1, Robim M Rodrigues, Maurice P Whelan
1Institute for Health and Customer Protection, European Commission Joint Research Centre, 21-027 Ispra (VA), Italy. a.bednarkiewicz@int.pan.wroc.pl
Abstract:
A quantitative, non-destructive cellular autofluorescence based in vitro imaging assay has been developed and applied to study the cytotoxicity of Sodium Lauryl Sulfate (SLS) and HgCl2 on Balb/c 3T3 cells. A phenomenological double logistic model was proposed to quantify and relate the observed kinetic changes of fluorescence to the toxic potency of chemical compounds. This work forms the basis for cellular autofluorescence measurements in in vitro toxicity screening assays.
