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Updated: May 28, 2026

Protein Engineering by Yeast Surface Display
Published on: November 29, 2024
A discussion of molecular biology methods for protein engineering
Alexander Zawaira1, Anil Pooran, Samantha Barichievy
1Gene Expression and Biophysics Group, Synthetic Biology-ERA, CSIR Biosciences, Brummeria, Pretoria 0001, South Africa. azawaira@gmail.com
Abstract:
A number of molecular biology techniques are available to generate variants from a particular start gene for eventual protein expression. We discuss the basic principles of these methods in a repertoire that may be used to achieve the elemental steps in protein engineering. These include site-directed, deletion and insertion mutagenesis. We provide detailed case studies, drawn from our own experiences, packaged together with conceptual discussions and include an analysis of the techniques presented with regards to their uses in protein engineering.
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