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Updated: May 28, 2026

A Protocol for Real-time 3D Single Particle Tracking
10:16

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

Nanoscale three-dimensional single particle tracking.

Aurélie Dupont1, Don C Lamb

  • 1Department of Chemistry, Center for NanoScience (CeNS), Ludwig-Maximilians-Universität München, Butenandtstr. 5-13, D-81377, München, Germany.

Nanoscale
|October 1, 2011
PubMed
Summary
This summary is machine-generated.

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Three-dimensional single particle tracking (3D SPT) offers quantitative insights into biomolecular functions. A new orbital tracking method enhances precision for studying cellular processes and virus infections.

Area of Science:

  • Biophysics
  • Cell Biology
  • Microscopy

Background:

  • Single particle tracking (SPT) is crucial for understanding biomolecular dynamics.
  • Classical ensemble methods lack the mechanistic detail provided by tracking individual molecules.
  • Advancements in tracking technologies are increasing demands for higher precision and new capabilities.

Purpose of the Study:

  • To review recent advancements in 3D SPT techniques.
  • To present a modified orbital tracking system for enhanced 3D single particle tracking.
  • To demonstrate the system's capability in tracking viral infection pathways.

Main Methods:

  • Review of image-based and feedback approaches in 3D SPT.
  • Development of a modified orbital tracking system utilizing simultaneous dual z-plane intensity measurements.

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3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles
11:28

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

Published on: October 1, 2014

Related Experiment Videos

Last Updated: May 28, 2026

A Protocol for Real-time 3D Single Particle Tracking
10:16

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles
11:28

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

Published on: October 1, 2014

  • Simultaneous wide-field imaging for concomitant observation.
  • Application of the system to trace single virus particles.

    • Main Results:

    • The modified orbital tracking system achieves high precision: 5 nm in x-y and 7 nm in the axial direction.
    • Demonstrated capability in tracking the infection pathway of Prototype Foamy Virus in living cells.
    • The technique provides quantitative mechanistic information on diffusional behavior and molecular interactions.

    Conclusions:

    • 3D SPT is an invaluable tool for investigating cellular processes.
    • The presented modified orbital tracking system offers significant improvements in tracking precision.
    • This technology enables detailed studies of biological pathways, such as viral infections.