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Related Experiment Video

Updated: May 28, 2026

Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation
10:52

Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation

Published on: January 6, 2016

Measuring PI3K lipid kinase activity.

Elisa Ciraolo1, Alessia Perino, Emilio Hirsch

  • 1Department of Genetics, Biology and Biochemistry, Molecular Biotechnology Center, University of Torino, Torino, Italy.

Methods in Molecular Biology (Clifton, N.J.)
|October 1, 2011
PubMed
Summary
This summary is machine-generated.

Class IA phosphoinositide-3 kinases (PI3Ks) are crucial in cancer survival and proliferation. This study details in vitro methods to measure PI3K lipid kinase activity, essential for testing new cancer drug efficacy.

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Last Updated: May 28, 2026

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PIP-on-a-chip: A Label-free Study of Protein-phosphoinositide Interactions

Published on: July 27, 2017

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Class IA phosphoinositide-3 kinases (PI3Ks) signaling is integral to cellular survival and proliferation.
  • Aberrant PI3K pathway activation is common in many cancers, correlating with resistance to conventional chemotherapy.
  • The PI3K pathway's role in cancer cell growth validates PI3K as a significant therapeutic target.

Purpose of the Study:

  • To present methodologies for assessing PI3K lipid kinase activity in vitro.
  • To provide a standard procedure for evaluating the efficacy of novel PI3K inhibitors.

Main Methods:

  • In vitro assays to measure PI3K lipid kinase activity.
  • Standardized protocols for inhibitor efficacy testing.

Main Results:

  • Established methods for quantifying PI3K lipid kinase activity.
  • Validated procedures for screening PI3K inhibitors.

Conclusions:

  • Accurate measurement of PI3K activity is critical for cancer drug development.
  • In vitro kinase assays are fundamental for advancing PI3K-targeted cancer therapies.