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Updated: May 28, 2026
A Protein Microarray Assay for Serological Determination of Antigen-specific Antibody Responses Following Clostridium difficile Infection
Published on: June 15, 2018
Proteomic analysis of sera from common variable immunodeficiency patients undergoing replacement intravenous
Giuseppe Spadaro1, Concetta D'Orio, Arturo Genovese
1Dipartimento di Medicina Clinica, Scienze Cardiovascolari e Immunologiche e Centro Interdipartimentale di Ricerca in Immunologia di Base e Clinica, Università degli Studi di Napoli Federico II, Via S. Pansini 5, 80131 Napoli, Italy. spadaro@unina.it
Abstract:
Common variable immunodeficiency is the most common form of symptomatic primary antibody failure in adults and children. Replacement immunoglobulin is the standard treatment of these patients. By using a differential proteomic approach based on 2D-DIGE, we examined serum samples from normal donors and from matched, naive, and immunoglobulin-treated patients. The results highlighted regulated expression of serum proteins in naive patients. Among the identified proteins, clusterin/ApoJ serum levels were lower in naive patients, compared to normal subjects. This finding was validated in a wider collection of samples from newly enrolled patients. The establishment of a cellular system, based on a human hepatocyte cell line HuH7, allowed to ascertain a potential role in the regulation of CLU gene expression by immunoglobulins.
