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Related Experiment Video

Updated: May 28, 2026

Primer for Immunohistochemistry on Cryosectioned Rat Brain Tissue: Example Staining for Microglia and Neurons
07:30

Primer for Immunohistochemistry on Cryosectioned Rat Brain Tissue: Example Staining for Microglia and Neurons

Published on: May 12, 2015

Highlighting anatomical sub-structures in rat brain tissue using lipid imaging.

Alice M Delvolve1, Benoit Colsch, Amina S Woods

  • 1Cellular Neurobiology, NIDA IRP, NIH, 333 Cassell Drive, Room 1120, Baltimore, MD, 21224, USA.

Analytical Methods : Advancing Methods and Applications
|October 1, 2011
PubMed
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Researchers developed a fast method using MALDI mass spectrometry to map specific lipids, phosphatidylcholines and sphingomyelins, in rat brain tissue. This technique aids in discovering the functions of lipids in cellular life.

Area of Science:

  • Neuroscience
  • Biochemistry
  • Analytical Chemistry

Background:

  • Cell membranes are complex lipid mixtures crucial for cellular functions.
  • Many lipid roles within cells remain undiscovered.
  • Accurate localization of lipids is essential for understanding their functions.

Purpose of the Study:

  • To develop an efficient, easy, and rapid methodology for lipid localization.
  • To accurately profile and image phosphatidylcholines and sphingomyelins in rat brain sections.
  • To advance the discovery of lipid functions in cellular biology.

Main Methods:

  • Matrix assisted laser desorption/ionization (MALDI) mass spectrometry was employed.
  • The method was applied to a single coronal rat brain section (cerebrum).

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Last Updated: May 28, 2026

Primer for Immunohistochemistry on Cryosectioned Rat Brain Tissue: Example Staining for Microglia and Neurons
07:30

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Published on: May 12, 2015

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  • Lipid profiling and imaging were performed to identify specific lipid species.

    • Main Results:

    • The methodology successfully localized phosphatidylcholines and sphingomyelins.
    • High-resolution lipid imaging was achieved with a best-resolved structure of 25-50 micrometers.
    • The hippocampus region showed the best structural resolution.

    Conclusions:

    • The developed MALDI mass spectrometry method is effective for precise lipid localization in brain tissue.
    • This technique facilitates the study of lipid distribution and function in the central nervous system.
    • The findings contribute to a deeper understanding of lipid roles in cellular processes.