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Updated: May 28, 2026
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A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions
Published on: June 6, 2018
[Screening PIAS2-interacting proteins in the mouse using the yeast two-hybrid system]
Ying Zheng1, Xiao-Qin Jia, Lu-Ping Zhang
1Department of Histology and Embryology, School of Medicine, Yangzhou University, Yangzhou, Jiangsu 225001, China. yzzkl@163.com
Zhonghua Nan Ke Xue = National Journal of Andrology
|October 4, 2011
Summary
Researchers identified eight proteins interacting with PIAS2 using yeast two-hybrid screening. These PIAS2-interacting proteins may play a role in regulating male fertility and spermatogenesis.
Area of Science:
- Molecular Biology
- Reproductive Biology
- Protein-Protein Interactions
Context:
- Spermatogenesis is a complex process crucial for male fertility.
- Protein inhibitor of activated STAT2 (PIAS2) is implicated in various cellular functions.
- Understanding PIAS2's interactions is key to elucidating its role in male reproduction.
Purpose:
- To identify novel proteins that interact with PIAS2 in mouse spermatogonia.
- To investigate the functional mechanisms of PIAS2 during spermatogenesis.
Summary:
- The yeast two-hybrid system was employed to screen a mouse spermatogonial cDNA library against PIAS2.
- Eight distinct proteins, including Cyfip2, Psmb3, Nmel, nischarin, Ints10, Nsun5, Gnb211, and Ndufaf3, were identified as PIAS2 interactors.
- Bioinformatic analysis and yeast two-hybrid verification confirmed these interactions.
Impact:
- This study successfully identified eight novel PIAS2-interacting proteins.
- The findings provide new insights into the molecular mechanisms governing PIAS2 function in spermatogenesis.
- These results may contribute to understanding the regulation of male fertility.
