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Updated: May 28, 2026

10:34
FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors
Published on: August 20, 2012
[cAMP, cGMP and their visualization in living cells using fluorescent microscopy]
Tsitologiia
|October 4, 2011
Abstract:
cAMP and cGMP are ubiquitous second messengers regulating a myriad of intracellular functions. Standard biochemical techniques to measure their levels in cells and tissues lack high temporal and any spatial resolution. To enable real-time monitoring of cAMP and cGMP in living cells and physiological systems, we and others have developed several biosensors based on fluorescence resonance energy transfer. This review will describe such novel techniques and discuss their application for various biological questions.

