Related Experiment Video
Updated: May 28, 2026
10:17
Rapid Determination of Antibody-Antigen Affinity by Mass Photometry
Published on: February 8, 2021
High-throughput analysis of concentration-dependent antibody self-association
Shantanu V Sule1, Muppalla Sukumar, William F Weiss
1Center for Biotechnology and Interdisciplinary Studies, Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Troy, New York, USA.
Biophysical Journal
|October 4, 2011
Summary
A new nanoparticle-based method rapidly measures antibody self-association. This technique reveals complex, specific interactions influenced by pH and ionic strength, guiding antibody behavior manipulation.
Area of Science:
- Biophysics
- Protein Chemistry
- Nanotechnology
Background:
- Monoclonal antibodies exhibit concentration-dependent self-association and viscosity.
- Traditional biophysical methods lack high-throughput capability for measuring antibody self-interactions.
Purpose of the Study:
- To develop and validate a nanoparticle-based method for high-throughput measurement of antibody self-association.
- To characterize the concentration-dependent self-interaction behavior of human monoclonal antibodies.
Main Methods:
- Self-interaction nanoparticle spectroscopy (SINS) using antibody-conjugated gold nanoparticles.
- Measurement of interparticle distance-dependent plasmon wavelength.
- Correlation with static light-scattering measurements.
Main Results:
- SINS accurately measures antibody self-association at low concentrations (<40 μg/mL).
- Antibody self-association is pH-dependent and influenced by ionic strength.
- Polyclonal antibodies showed no self-association, indicating specificity.
Conclusions:
- Nanoparticle-based spectroscopy offers a rapid, high-throughput method for studying antibody self-association.
- Findings provide insights into factors governing antibody phase behavior.
- Results suggest antibody self-association is a specific phenomenon.
