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Applications of Liquid-Chromatography Tandem Mass Spectrometry in Natural Products Research: Tropane Alkaloids as a Case Study
Published on: March 8, 2024
Antiophidic solanidane steroidal alkaloids from Solanum campaniforme
Maria Conceição M Torres1, Francisco das Chagas L Pinto, Raimundo Braz-Filho
1Departamento de Química Orgânica e Inorgânica, Universidade Federal do Ceará, 12.200, Fortaleza-CE, 60.021-970, Brazil.
Abstract:
Three new solanidane alkaloids bearing a 22,23-epoxy ring (1-3) and four known compounds were isolated from leaves of Solanum campaniforme. The structures were determined using spectroscopic techniques, including 1D and 2D NMR, and HRESIMS experiments. The antiophidic activity of the alkaloids was tested against Bothrops pauloensis venom. Compounds 1-3 completely inhibited myotoxicity without inhibiting phospholipase A2 activity of the venom, while hemorrhage and skin necrosis were significantly reduced in the presence of alkaloids 1 and 2.
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