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Distribution of extended-spectrum beta-lactamase genes using a commercial DNA micro-array system
R H T Nijhuis1, A A van Zwet, P H M Savelkoul
1Department of Medical Microbiology and Medical Immunology, Rijnstate, Velp, The Netherlands. rnijhuis@rijnstate.nl
The Journal of Hospital Infection
|October 4, 2011
Summary
Extended-spectrum beta-lactamase (ESBL) gene distribution was analyzed in Dutch clinical isolates. CTX-M-1 and CTX-M-9 groups were most common, with variations seen between hospitalized and outpatient groups.
Area of Science:
- Microbiology
- Infectious Diseases
- Genetics
Background:
- Extended-spectrum beta-lactamase (ESBL) genes are a growing global health concern.
- Understanding the epidemiology of ESBL genes is crucial for effective treatment and control.
Purpose of the Study:
- To determine the distribution of class A ESBL genes in clinical isolates of aerobic Gram-negative bacilli.
- To analyze ESBL gene prevalence across different patient categories (ICU, non-ICU inpatients, outpatients).
Main Methods:
- Utilized the Check-ESBL assay to detect ESBL genes in isolates identified by automated systems (Vitek2, Phoenix).
- Categorized 491 putative ESBL-positive isolates from three laboratories in the East of The Netherlands (February 2009–March 2010).
- Analyzed 274 identified ESBL genes from 247 confirmed ESBL-positive isolates.
Main Results:
- ESBL genes were detected in 50.3% of putative isolates (247/491).
- CTX-M-1 group (153 genes) and CTX-M-9 group (67 genes) were the most prevalent.
- ESBL-producing E. cloacae were more frequent in hospitalized patients (20.7%) vs. outpatients (3.8%) (P=0.001).
- CTX-M-9 group ESBLs were more prevalent in ICU patients (P=0.003), and SHV ESBLs in hospitalized patients (P<0.001).
Conclusions:
- Significant differences in ESBL gene distribution exist between hospitalized and outpatient populations.
- Specific ESBL types show distinct prevalence patterns in intensive care unit patients.
- The study highlights the complex epidemiology of ESBL genes in the Netherlands.
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