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Related Concept Videos

Thermosensation01:43

Thermosensation

Peripheral thermosensation is the perception of external temperature. A change in temperature (on the surface of the skin and other tissues) is detected by a family of temperature-sensitive ion channels called Transient Receptor Potential, or TRP, receptors. These receptors are located on free nerve endings. Those detecting cold temperatures are closer to the surface of the skin than the nerve endings detecting warmth. These thermoTRP channels, while temperature selective, have relatively...
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When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
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Related Experiment Video

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Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale
08:49

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Published on: May 28, 2021

TRP-channel-specific cutaneous eicosanoid release patterns.

Anil Jain1, Simone Brönneke, Ludger Kolbe

  • 1Research and Development, Beiersdorf AG, Hamburg, Germany.

Pain
|October 4, 2011
PubMed
Summary
This summary is machine-generated.

Non-neuronal skin cells express functional TRPV1 and TRPA1 channels. Stimulation triggers differential inflammatory mediator release and pain sensations, impacting skin conditions and pain management.

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Last Updated: May 28, 2026

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Purification and Reconstitution of TRPV1 for Spectroscopic Analysis

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Area of Science:

  • Dermatology
  • Neuroscience
  • Molecular Biology

Background:

  • Peripheral nociception research increasingly focuses on non-neuronal skin cells.
  • Transient Receptor Potential (TRP) channels, including TRPV1 and TRPA1, are implicated in pain and inflammation.

Purpose of the Study:

  • Investigate the functional expression of TRPV1 and TRPA1 in human keratinocytes and fibroblasts.
  • Assess the release of proinflammatory lipid mediators and sensory effects upon channel stimulation.
  • Combine in vitro and in vivo approaches to understand these mechanisms.

Main Methods:

  • Confirmed functional TRPV1 and TRPA1 expression using immunofluorescence, RT-PCR, and calcium influx assays.
  • Stimulated cells with TRPA1 agonist allyl isothiocyanate (AITC) and TRPV1 agonist capsaicin.
  • Analyzed eicosanoid (PGE2, LTB4) release in vitro and in suction blister fluid in vivo.
  • Assessed sensory effects like heat pain thresholds and erythema after topical application.

Main Results:

  • Both keratinocytes and fibroblasts express functional TRPV1 and TRPA1 channels.
  • Capsaicin (TRPV1) induced LTB4 release and decreased PGE2 levels.
  • AITC (TRPA1) increased PGE2 levels at 24 hours but did not affect LTB4.
  • Both agonists reduced heat pain thresholds; AITC caused prolonged erythema.
  • Differential mediator release patterns were observed in vitro and confirmed in vivo.

Conclusions:

  • TRPV1 and TRPA1 activation in non-neuronal cells elicits pain and differential inflammatory mediator release.
  • These mediators influence local vasodilation and neuronal sensitization.
  • Findings suggest potential therapeutic applications for TRP channel antagonists in inflammatory skin conditions and pain management.