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Exploratory study of proteins in urine of patients with histoplasma antigenuria

Mark M Kushnir1, David K Crockett, Joann L Cloud

  • 1ARUP® Institute for Clinical and Experimental Pathology, Salt Lake City, UT 84108, United States. kushnmm@aruplab.com

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
|October 4, 2011
PubMed

Insights

This study explored urinary protein differences in healthy individuals, proteinuria (PRU) patients, and histoplasma antigenuria (HIS) patients. It identified unique proteins and pathways in PRU and HIS, aiding future research on these conditions.

Area of Science:

  • * Proteomics
  • * Mycology
  • * Immunology

Background:

  • * Disseminated histoplasmosis is a severe fungal infection impacting immunocompromised individuals.
  • * Urinary protein profiling offers insights into disease states.
  • * Proteinuria (PRU) and histoplasma antigenuria (HIS) represent distinct urinary conditions.

Purpose of the Study:

  • * To compare urinary protein profiles between healthy individuals, PRU patients, and HIS patients.
  • * To identify proteins unique to PRU and HIS patient groups.
  • * To elucidate physiological pathways associated with proteins found in PRU and HIS.

Main Methods:

  • * Urine samples underwent abundant protein depletion and deglycosylation.
  • * Proteins were digested, fractionated, and analyzed using nano-LC-QTOF.
  • * Identified proteins were subjected to pathway analysis.

Main Results:

  • * A total of 117 human proteins were identified across all samples.
  • * 20 proteins were unique to PRU samples, and 23 proteins were unique to HIS samples.
  • * Pathway analysis revealed associations with acute response, coagulation, and lipid antigen presentation in PRU and HIS.

Conclusions:

  • * Distinct urinary protein signatures exist for proteinuria and histoplasma antigenuria.
  • * Identified pathways offer potential biomarkers and therapeutic targets.
  • * The study demonstrates the feasibility of rigorous proteomic analysis for proteinuria research.

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