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Related Experiment Video

Updated: May 28, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Gas-phase purification enables accurate, multiplexed proteome quantification with isobaric tagging.

Craig D Wenger1, M Violet Lee, Alexander S Hebert

  • 1Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA.

Nature Methods
|October 4, 2011
PubMed
Summary

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QuantMode, a new mass spectrometry method, enhances quantitative proteomics accuracy by purifying precursor ions, reducing interference from contaminants. This gas-phase purification technique improves data reliability for large-scale multiplexed studies.

Area of Science:

  • Proteomics
  • Analytical Chemistry
  • Biochemistry

Background:

  • Isobaric tag-based quantification is crucial for comparing protein levels across samples.
  • Precursor interference, caused by co-isolated and co-fragmented species, significantly compromises quantitative accuracy.
  • Existing methods struggle to effectively remove these interfering ions during mass spectrometry analysis.

Purpose of the Study:

  • To introduce QuantMode, a novel mass spectrometry method for improved isobaric tag-based quantification.
  • To address and mitigate the challenge of precursor interference in quantitative proteomics.
  • To enable more accurate and reliable large-scale multiplexed quantitative proteomics.

Main Methods:

  • Development of QuantMode, a gas-phase purification technique integrated into mass spectrometry.

More Related Videos

A Quantitative Glycomics and Proteomics Combined Purification Strategy
11:38

A Quantitative Glycomics and Proteomics Combined Purification Strategy

Published on: March 8, 2016

Related Experiment Videos

Last Updated: May 28, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

A Quantitative Glycomics and Proteomics Combined Purification Strategy
11:38

A Quantitative Glycomics and Proteomics Combined Purification Strategy

Published on: March 8, 2016

  • Application of QuantMode to analyze a yeast sample spiked with interfering human peptides.
  • Comparison of quantitative accuracy and spectral identification rates between QuantMode and standard mass spectrometry scans.

    • Main Results:

    • QuantMode significantly improved the quantitative accuracy of isobaric tag-based measurements in the presence of interfering peptides.
    • The method demonstrated enhanced data reliability by reducing the impact of contaminants.
    • A minor reduction in the number of spectral identifications was observed, with a substantial gain in accuracy.

    Conclusions:

    • QuantMode effectively alleviates precursor interference in isobaric tag-based quantification.
    • This technique offers a robust solution for accurate, large-scale, multiplexed quantitative proteomics.
    • QuantMode represents a significant advancement for high-throughput proteomic analyses.