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Related Concept Videos

Phosphoinositides and PIPs01:42

Phosphoinositides and PIPs

Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview01:19

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then passed on to...

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Related Experiment Video

Updated: May 28, 2026

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry
08:07

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

Published on: July 26, 2019

Methods for analyzing phosphoinositides using mass spectrometry.

Michael J O Wakelam1, Jonathan Clark

  • 1The Babraham Institute, Cambridge CB22 3AT, UK. Michael.wakelam@bbsrc.ac.uk

Biochimica Et Biophysica Acta
|October 4, 2011
PubMed
Summary
This summary is machine-generated.

This review discusses polyphosphoinositides (PPIs), crucial signaling lipids. Current mass spectrometry (MS) methods struggle to accurately quantify distinct PPI molecular species due to extraction and binding challenges.

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Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation
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Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation

Published on: January 6, 2016

A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors
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A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors

Published on: April 29, 2022

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Last Updated: May 28, 2026

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry
08:07

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

Published on: July 26, 2019

Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation
10:52

Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation

Published on: January 6, 2016

A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors
10:17

A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors

Published on: April 29, 2022

Area of Science:

  • Cellular lipid signaling
  • Molecular biology
  • Biochemistry

Background:

  • Polyphosphoinositides (PPIs) are essential signaling lipids with tightly regulated cellular levels.
  • Multiple PPI species exist, differing in acyl chain composition, which impacts their function.
  • Existing analytical methods often analyze deacylated derivatives, failing to distinguish between distinct PPI molecular forms.

Purpose of the Study:

  • To review available mass spectrometry (MS) methodologies for polyphosphoinositide analysis.
  • To highlight the specific challenges and limitations associated with each MS-based technique.
  • To assess the current state of quantitative lipidomics for PPIs.

Main Methods:

  • Review of existing literature on mass spectrometry (MS) techniques for lipidomic analysis.
  • Discussion of challenges including lipid extraction efficiency and surface binding artifacts.
  • Comparative analysis of different MS approaches for PPI quantification.

Main Results:

  • Various MS methodologies for PPI analysis are available, each with inherent difficulties.
  • Extraction and lipid binding issues significantly hamper accurate lipidomic analysis of PPIs.
  • No single current methodology can reliably and reproducibly quantify all inositol phospholipid species.

Conclusions:

  • Accurate and reproducible quantification of distinct polyphosphoinositide molecular species remains a significant challenge in lipidomics.
  • Further development of MS methodologies is required to overcome extraction and binding limitations.
  • Improved analytical techniques are crucial for understanding PPI signaling pathways.