Related Experiment Video
Updated: May 28, 2026

Rapid Quantification of Oxidized and Reduced Forms of Glutathione Using Ortho -phthalaldehyde in Cultured Mammalian Cells In Vitro
Published on: June 28, 2024
Voltammetric determination of glutathione in haemolysed erythrocyte and tablet samples using modified-multiwall
Ali A Ensafi1, Samira Dadkhah-Tehrani, Hassan Karimi-Maleh
1Department of Chemistry, Isfahan University of Technology, Iran. Ensafi@cc.iut.ac.ir
Abstract:
A chemically modified electrode was prepared by incorporating p-aminophenol into multiwall carbon nanotubes paste matrix. Cyclic voltammetry, square wave voltammetry, double potential step chronoamperometry, and electrochemical impedance spectroscopy were used to investigate the electrochemical behaviour of glutathione at the chemically modified electrode prepared. According to the results, p-aminophenol multiwall carbon nanotubes paste electrode (p-APMWCNTPE) showed high electrocatalytic activity for glutathione oxidation, producing a sharp oxidation peak current at about +0.285 vs Ag/AgCl reference electrode at pH 5.0. Chronoamperometry was also used to determine glutathione's catalytic rate constant and diffusion coefficient at p-APMWCNTPE. The square wave voltammetric peak current of glutathione increased linearly with glutathione concentration in the range of 2.0 × 10(-7) - 1.0 × 10(-4) mol L(-1) with a detection limit of 9.0 × 10(-8) mol L(-1). The method was also successfully employed as a selective, simple, and precise method for the determination of glutathione in haemolysed erythrocyte, tablet, and urine samples.
Related Concept Videos
Voltammetry: Stripping Methods
Anodic Stripping Voltammetry (ASV)
ASV is used to determine metals and metalloids at trace levels. It involves two steps: deposition and stripping. First, a negative potential is applied to the...
Voltammetric Techniques: Cyclic Voltammetry
Voltammetry: Overview
A voltammetric cell uses three electrodes: a working electrode, a reference electrode, and an auxiliary electrode. The redox reactions occur in the working...
Electrogravimetric Analysis: Overview
To test the completeness of the...

