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Cryo-electron Microscopy

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...

Fixation and Sectioning

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...

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Related Experiment Video

Updated: May 28, 2026

Cryosectioning of Contiguous Regions of a Single Mouse Skeletal Muscle for Gene Expression and Histological Analyses

Cryosectioning of Contiguous Regions of a Single Mouse Skeletal Muscle for Gene Expression and Histological Analyses

Published on: December 12, 2016

Cryosectioning.

¹University of Pittsburgh Medical School, Pittsburgh, Pennsylvania, USA.

Current Protocols in Pharmacology

|October 4, 2011

Summary

This summary is machine-generated.

This guide details frozen tissue sample preparation and sectioning for microscopy. These techniques are crucial for various light microscopic analyses, including in situ hybridization and immunohistochemistry.

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Last Updated: May 28, 2026

Cryosectioning of Contiguous Regions of a Single Mouse Skeletal Muscle for Gene Expression and Histological Analyses

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Published on: December 12, 2016

Flash Freezing and Cryosectioning E12.5 Mouse Brain

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Cryosectioning Method for Microdissection of Murine Colonic Mucosa

Cryosectioning Method for Microdissection of Murine Colonic Mucosa

Published on: July 12, 2015

Area of Science:

Histology and Microscopy
Biotechnology

Background:

Accurate tissue sample preparation is fundamental for reliable microscopic analysis.
Frozen tissue sectioning preserves cellular morphology and antigenicity for advanced techniques.

Purpose of the Study:

To describe standardized methods for preparing and sectioning frozen tissue samples.
To provide a foundational protocol for subsequent light microscopic procedures.

Main Methods:

Sample preparation techniques for fresh-frozen tissues.
Cryosectioning methodologies for obtaining thin tissue slices.
Mounting and handling of frozen tissue sections.

Main Results:

Successfully prepared frozen tissue sections suitable for various staining protocols.
Demonstrated the utility of these sections in immunohistochemistry and enzyme histochemistry.

Conclusions:

Standardized frozen tissue sectioning is essential for high-quality light microscopy.
These methods support diverse applications in diagnostic and research histology.