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Nonisotopic assays for reporter gene activity

A R Brasier1, J J Fortin

  • 1University of Texas, Galveston, Texas, USA.

Current Protocols in Pharmacology
|October 4, 2011
PubMed

Abstract:

This unit describes two nonisotopic systems for reporter gene activity in cells transfected with the firefly luciferase.

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Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

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