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Identification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor Libraries
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Determining signalling nodes for apoptosis by a genetic high-throughput screen.

Bevan Lin1, Derek Huntley, Ghada Abuali

  • 1Division of Experimental Medicine, Imperial College London, London, United Kingdom.

Plos One
|October 4, 2011
PubMed
Summary

Researchers identified 74 novel genes that trigger apoptosis, or programmed cell death, revealing new insights into this complex cellular process. This discovery enhances our understanding of cell suicide regulation and potential disease links.

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Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death

Published on: December 27, 2016

Area of Science:

  • Genetics
  • Molecular Biology
  • Cell Biology

Background:

  • Increasing genomic data necessitates understanding novel gene functions.
  • Apoptosis (programmed cell death) is a complex process regulated by numerous factors.

Purpose of the Study:

  • To identify novel genes that induce apoptosis using a high-throughput screening method.
  • To characterize the functional roles and distribution of newly discovered apoptosis regulators.

Main Methods:

  • A high-throughput screen was developed using 96-well microtiter plates for gene transfection.
  • Approximately 100,000 cDNA clones were screened to identify apoptosis-inducing genes.
  • Bioinformatics and sequence analysis were performed on identified genes.

Main Results:

  • 74 genes were identified that initiate apoptosis upon transfection into human cells.
  • 91% of the identified genes are novel apoptosis regulators.
  • Apoptosis factors showed distinct functional distributions (transporters, enzymes) compared to other signaling pathways.
  • Identified genes are located in various cellular organelles and signaling circuits, with some connecting via a distinct pathway.
  • Several identified genes are potentially dysregulated in cancers and degenerative diseases.

Conclusions:

  • The screen revealed numerous unknown genes involved in cell death, highlighting the complexity of apoptosis regulation.
  • These findings provide a valuable resource for researchers studying genomics, apoptosis, and cell suicide signaling pathways.