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Angiopoietin-like 4 regulates epidermal differentiation
Mintu Pal1, Ming Jie Tan, Royston-Luke Huang
1School of Biological Sciences, College of Science, Nanyang Technological University, Singapore, Singapore.
Plos One
|October 4, 2011
Summary
Peroxisome proliferator-activated receptor beta/delta (PPARβ/δ) promotes skin epidermal maturation by regulating angiopoietin-like 4 (ANGPTL4) expression, which is crucial for keratinocyte differentiation and wound healing.
Area of Science:
- Dermatology
- Molecular Biology
- Cell Biology
Background:
- The nuclear hormone receptor PPARβ/δ plays a role in epidermal maturation.
- The precise mechanisms governing PPARβ/δ-mediated epidermal differentiation are not fully understood.
Purpose of the Study:
- To elucidate the mechanism by which PPARβ/δ regulates epidermal differentiation.
- To investigate the role of angiopoietin-like 4 (ANGPTL4) in PPARβ/δ-induced keratinocyte differentiation.
Main Methods:
- Organotypic skin cultures from knockdown keratinocytes
- Real-time PCR gene expression analysis
- Chromatin immunoprecipitation assays
Main Results:
- Ligand-activated PPARβ/δ indirectly stimulates keratinocyte differentiation via de novo gene transcription and protein translation.
- ANGPTL4, a PPARβ/δ target gene, is essential for this differentiation process.
- PPARβ/δ activation influences protein kinase C isotypes and phosphorylated activator protein-1, impacting epidermal differentiation markers like involucrin and transglutaminase type 1.
Conclusions:
- PPARβ/δ regulates epidermal maturation through an ANGPTL4-mediated signaling pathway.
- ANGPTL4 is a key mediator in PPARβ/δ-driven keratinocyte differentiation and epidermal development.
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