Related Experiment Video
Updated: May 28, 2026

Biogas Purification through the use of a Microalgae-Bacterial System in Semi-Industrial High Rate Algal Ponds
Published on: March 22, 2024
The optimization of biohydrogen production by bacteria using residual glycerol from biodiesel synthesis
Janaina B Costa1, Daniele M Rossi, Elisangela A De Souza
1Department of Chemistry, Universidade Federal do Rio Grande do Sul, Brazil.
Abstract:
In this research the production of hydrogen by Klebsiella pneumoniae BLb01 using residual glycerol discharged from a biodiesel fuel production plant was investigated. Klebsiella pneumoniae BLb01 was isolated from a bacteria-rich sludge of an upflow anaerobic sludge blanket reactor (UASB) of a soybean processing plant. A Plackett-Burman design (P-B) and Response Surface Methodology (RSM) were employed to determine the optimal condition for enhanced hydrogen production. The maximal hydrogen production, which was 45.0 mol % and with 98% of glycerol degradation, was achieved with the optimized medium with the following composition: 30 g L(-1) glycerol; 3 g L(-1) yeast ex tract 3 g L(-1) K(2)HPO(4); 1 g L(-1) KH(2)PO(4); temperature 39°C and pH 9.0. These results show the ability of this new strain of effectively converting residual glycerol into value-added energy products.
Related Concept Videos
Biofuels
Microbial Bioremediation of Hydrocarbons
Lipid Catabolism
Bioremediation
Production of Alcohol
Bioreactor Controls-III

