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A Multi-compartment CNS Neuron-glia Co-culture Microfluidic Platform
Published on: September 10, 2009
Microfluidic and compartmentalized platforms for neurobiological research
1Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill and North Carolina State University, Chapel Hill, NC 27599, USA. amtaylor@unc.edu
Critical Reviews in Biomedical Engineering
|October 5, 2011
Summary
Neuronal compartmentalization techniques, from early chambers to advanced microfluidic platforms, enable detailed study of neuron segments like axons and synapses in neurobiology research.
Area of Science:
- Neuroscience
- Cell Biology
Background:
- Neuronal compartmentalization isolates distinct cell parts (cell bodies, axons, dendrites, synapses).
- Traditional methods used machined dividers and grease layers for peripheral neurons.
- These methods enabled studies on axonal transport, injury, and regeneration.
Purpose of the Study:
- To review traditional and modern neuronal compartmentalization methods.
- To highlight the utility of microfluidic platforms for central nervous system (CNS) neuron culture.
- To discuss applications in studying axonal and synaptic biology.
Main Methods:
- Development of machined Teflon dividers with grease layers.
- Advancement to microfluidic platforms using microfabrication and soft lithography.
- Utilizing microgrooves within solid barriers for neuronal segment separation.
Main Results:
- Microfluidic platforms enable culturing of CNS neurons with high-resolution live imaging.
- These platforms facilitate novel investigations into axonal and synaptic biology.
- Improvements offered for other neural cell and tissue preparations.
Conclusions:
- Compartmentalization is crucial for studying neuronal function in vivo and in vitro.
- Microfluidic platforms represent a significant advancement for neurobiology research.
- These platforms are valuable for defining and manipulating synapses.
