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Related Experiment Video

Updated: May 28, 2026

A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters
12:14

A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters

Published on: March 9, 2016

Method repeatability for measuring Enterococcus in southern California beach sands.

Y Cao1, C D McGee, J F Griffith

  • 1Southern California Coastal Water Research Project, Costa Mesa, CA 92626, USA.

Letters in Applied Microbiology
|October 5, 2011
PubMed
Summary
This summary is machine-generated.

A simple method for counting fecal indicator bacteria in beach sand is easily transferable and repeatable for labs without prior experience. This supports method standardization for understanding sand

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Area of Science:

  • Environmental microbiology
  • Water quality monitoring
  • Bacteriology

Background:

  • Standardization of methods for enumerating fecal indicator bacteria in sand is crucial for accurate water quality assessment.
  • Previous studies recommended a preferred method, but its transferability to inexperienced laboratories was unknown.

Purpose of the Study:

  • To evaluate the transferability and repeatability of a recommended method for enumerating fecal indicator bacteria in sand to laboratories lacking extensive experience.
  • To assess the method's performance in diverse laboratory settings.

Main Methods:

  • Eight southern California laboratories with limited experience in sand sample processing participated.
  • Laboratories processed replicate sand and water samples using the recommended enumeration method.
  • Repeatability and among-laboratory variability were assessed.

Main Results:

  • Among-laboratory variability was lower than within-laboratory variability.
  • No significant differences in results were observed among the participating laboratories.
  • Within-laboratory variability for sand samples was comparable to water samples, indicating minimal additional error from the elution process.

Conclusions:

  • The simple extraction method for enumerating Enterococcus in beach sands demonstrated easy transferability and repeatability.
  • The findings support successful technology transfer and method standardization for improved water quality monitoring.
  • This facilitates a better understanding of sand's impact on surface water quality.