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Related Concept Videos

Formation of the Platelet Plug01:22

Formation of the Platelet Plug

The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
Structure and Function of Platelets01:18

Structure and Function of Platelets

The cell fragments known as platelets are disc-shaped, with an average diameter of about 3 μm and a thickness of roughly 1 μm. They play a crucial role in the body's vascular clotting system, which also involves plasma proteins, blood cells, and blood vessel tissues.
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000 platelets, with...
Antiplatelet Drugs: Prostaglandin Synthesis, P2Y12 and Glycoprotein IIb/IIIa Inhibitors01:20

Antiplatelet Drugs: Prostaglandin Synthesis, P2Y12 and Glycoprotein IIb/IIIa Inhibitors

Antiplatelet drugs emerge as frontline defenders against the insidious threat of thromboembolic diseases, where abnormal clots obstruct vital blood vessels. These drugs stand as bulwarks, inhibiting platelet aggregation and clot formation, thereby mitigating the risk of life-threatening conditions like myocardial infarction, coronary artery disease, and thrombotic strokes.
Prostaglandin synthesis inhibitors, exemplified by the widely known aspirin, wield their power by irreversibly acetylating...

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Updated: May 28, 2026

Analyzing Platelet Subpopulations by Multi-color Flow Cytometry
08:04

Analyzing Platelet Subpopulations by Multi-color Flow Cytometry

Published on: June 10, 2025

NAADP regulates human platelet function.

Carmen H Coxon1, Alexander M Lewis, Amanda J Sadler

  • 1Department of Physiology, Anatomy and Genetics, University of Oxford, Le Gros Clark Building, South Parks Road, Oxford OX1 3QX, U.K. carmen.coxon@dpag.ox.ac.uk

The Biochemical Journal
|October 5, 2011
PubMed
Summary
This summary is machine-generated.

Nicotinic acid-adenine dinucleotide phosphate (NAADP) is crucial for human platelet activation. A new antagonist, Ned-19, blocks NAADP receptors, inhibiting platelet responses and offering potential anti-thrombotic therapies.

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04:37

Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation

Published on: May 23, 2025

Area of Science:

  • Biochemistry
  • Cell Biology
  • Pharmacology

Background:

  • Platelets are essential for hemostasis, and their activation involves calcium (Ca2+) release.
  • Nicotinic acid-adenine dinucleotide phosphate (NAADP) is a key Ca2+-releasing second messenger acting on acidic Ca2+ stores.
  • Previous studies indicated NAADP's role in permeabilized human platelets and thrombin-mediated activation.

Purpose of the Study:

  • To further characterize NAADP-mediated Ca2+ release in human platelets.
  • To investigate the role of NAADP in response to thrombin and collagen-related peptide (CRP) agonists.
  • To identify and characterize the NAADP receptor in human platelets.

Main Methods:

  • Radioligand-binding assay to detect NAADP-binding sites.
  • Measurement of 45Ca2+ release from intracellular stores.
  • Assessment of the effects of NAADP and the antagonist Ned-19 on platelet function (activation, aggregation, secretion, spreading).
  • Evaluation of Ned-19's effect on whole blood clotting time.

Main Results:

  • A specific NAADP-binding site was identified in human platelets, suggesting the presence of an NAADP receptor.
  • NAADP induced Ca2+ release from intracellular stores, inhibited by nigericin.
  • The NAADP antagonist Ned-19 competed for the binding site and inhibited thrombin- and CRP-induced Ca2+ release.
  • Ned-19 demonstrated inhibitory effects on platelet aggregation, secretion, spreading, and prolonged whole blood clotting time.

Conclusions:

  • NAADP plays a significant role in regulating human platelet function.
  • The NAADP receptor is present and functional in human platelets.
  • Ned-19, as an NAADP receptor antagonist, shows therapeutic potential for platelet-targeted therapies and thrombosis regulation.