Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Insulin enhancer binding protein has helix-loop-helix structure.

Y Shibasaki1, H Sakura, F Takaku

  • 1Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.

Biochemical and Biophysical Research Communications
|July 16, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Serum erythropoietin titers in hematological malignancies and related diseases.

International journal of cell cloning·1992
Same author

Increased respiratory burst activity of neutrophils in patients with aplastic anemia: effects of granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor.

Experimental hematology·1992
Same author

Production of interleukin 3 and granulocyte-macrophage colony-stimulating factor from stimulated blood mononuclear cells in patients with aplastic anemia.

Experimental hematology·1992
Same author

Correlation of antibodies to ribosomal P protein with psychosis in patients with systemic lupus erythematosus.

Annals of the rheumatic diseases·1992
Same author

Changes of enzyme activity levels in red blood cells in hemodialysis patients by recombinant human erythropoietin.

Nihon Jinzo Gakkai shi·1992
Same author

[Present status and future aspects of clinical use of cytokines].

Nihon rinsho. Japanese journal of clinical medicine·1992

Researchers identified a protein that binds to a specific DNA motif, regulating insulin gene expression in pancreatic B cells. This protein is similar to one that regulates immunoglobulin genes, suggesting a shared regulatory mechanism.

Area of Science:

  • Molecular Biology
  • Genetics
  • Endocrinology

Background:

  • Insulin gene expression is specific to pancreatic B cells.
  • The 5' flanking region of the insulin gene controls tissue specificity.
  • A GCCATCTG motif within this region acts as an enhancer for insulin transcription.

Purpose of the Study:

  • To identify the protein that binds to the GCCATCTG motif in the rat insulin 1 gene.
  • To understand the role of this binding protein in insulin gene regulation.

Main Methods:

  • Isolation of a cDNA encoding the GCCATCTG motif-binding protein (IEBP1) using a lambda gt11 library from a rat pancreatic B cell tumor.
  • Analysis of the IEBP1 protein's structure and function.

Main Results:

Related Experiment Videos

  • IEBP1 was isolated and identified as the rat counterpart of the E12/47 protein.
  • IEBP1 possesses a helix-loop-helix domain, characteristic of transcription factors.
  • This finding suggests that IEBP1 binds to the GCCATCTG enhancer motif.

Conclusions:

  • The immunoglobulin (Ig) enhancer binding protein E12/47 (IEBP1) is involved in insulin gene transcription.
  • Both the Ig gene and the insulin gene utilize the same or a similar binding protein for transcriptional regulation.
  • This highlights a potential shared regulatory mechanism between immune and endocrine genes.