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Assay Development for High-Throughput Drug Screening Against Mycobacteria
Published on: October 25, 2024
Bioenhancing and antimycobacterial agents from Ammannia multiflora.
Harish C Upadhyay1, Gaurav R Dwivedi, Mahendra P Darokar
1Analytical Chemistry Division, Central Institute of Medicinal and Aromatic Plants, P. O. CIMAP, Lucknow, India.
Planta Medica
|October 5, 2011
Summary
The methanolic extract of Ammannia multiflora enhances antibiotic efficacy. Bioassay-guided fractionation isolated ammaniol, with both the extract and isolated compounds showing significant bioenhancing activity against Escherichia coli.
Area of Science:
- Phytochemistry
- Pharmacology
- Medicinal Chemistry
Background:
- Investigating the bioenhancing potential of Ammannia multiflora extract in combination with nalidixic acid.
- Exploring the isolation and characterization of bioactive compounds from A. multiflora.
Discussion:
- The methanolic extract of A. multiflora demonstrated significant bioenhancing activity, reducing the required dose of nalidixic acid.
- Isolation of a novel compound, ammaniol (5), alongside nine known compounds from the plant extract.
- Evaluation of isolated compounds and semisynthetic derivatives for their ability to potentiate nalidixic acid against Escherichia coli strains.
Key Insights:
- Ammannia multiflora extract and compounds 1 and 9 exhibited notable bioenhancing effects, enabling a fourfold dose reduction of nalidixic acid.
- Compounds 5, 6, 10, and semisynthetic derivatives 1A-1E showed twofold dose reduction of nalidixic acid.
- Ammaniol (compound 5) displayed moderate antimycobacterial activity against Mycobacterium H37Rv (MIC 25 µg/mL).
Outlook:
- Further research into ammaniol and other isolated compounds for their potential as antibiotic adjuvants.
- Investigating the mechanism of bioenhancement by A. multiflora constituents.
- Exploring the therapeutic applications of ammaniol in treating mycobacterial infections.
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