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Related Experiment Video

Updated: May 28, 2026

miRNA Expression Analyses in Prostate Cancer Clinical Tissues
11:29

miRNA Expression Analyses in Prostate Cancer Clinical Tissues

Published on: September 8, 2015

Decrease in CD8+ lymphocyte number and altered cytokine profile in human prostate cancer.

Gallya Gannot, Annely M Richardson, Jaime Rodriguez-Canales

    American Journal of Cancer Research
    |October 5, 2011
    PubMed
    Summary
    This summary is machine-generated.

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    Prostate cancer alters the tumor microenvironment, decreasing CD8 positive T lymphocytes near cancerous and hyperplastic regions. Cytokine profiles also show significant changes at the mRNA level in affected prostate glands.

    Area of Science:

    • Oncology
    • Immunology
    • Cell Biology

    Background:

    • The tumor microenvironment (TME) is a complex ecosystem involving diverse cell types and their interactions.
    • Inflammatory responses within the TME are crucial in cancer development and progression.
    • Understanding cellular composition and molecular signaling in the prostate TME is vital for cancer research.

    Purpose of the Study:

    • To investigate the spatial distribution of lymphocyte subpopulations in the prostate TME.
    • To analyze the cytokine profiles within normal, pre-cancerous, and cancerous prostate tissues.
    • To correlate immune cell changes with molecular alterations in prostate cancer.

    Main Methods:

    • Quantitative imaging was employed to measure lymphocyte subpopulations (CD8+, CD4+, CD20+).

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    Isolation of Cancer Stem Cells From Human Prostate Cancer Samples
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    Isolation of Cancer Stem Cells From Human Prostate Cancer Samples

    Published on: March 14, 2014

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    miRNA Expression Analyses in Prostate Cancer Clinical Tissues
    11:29

    miRNA Expression Analyses in Prostate Cancer Clinical Tissues

    Published on: September 8, 2015

    Isolation of Cancer Stem Cells From Human Prostate Cancer Samples
    07:16

    Isolation of Cancer Stem Cells From Human Prostate Cancer Samples

    Published on: March 14, 2014

  • Expression microarray analysis was performed to assess mRNA levels of cytokines.
  • Analysis encompassed four epithelial phenotypes: carcinoma, prostatic intraepithelial neoplasia (PIN), benign prostate hyperplasia (BPH), and normal epithelium.

    • Main Results:

    • CD8 positive, cytotoxic T lymphocytes were significantly reduced in areas adjacent to carcinoma and BPH compared to normal and PIN tissues.
    • CD4 positive and CD20 positive lymphocyte populations showed no significant changes.
    • Expression microarray revealed distinct cytokine profiles in cancer, with 24 dysregulated genes in tumor epithelium and nine in tumor-associated stroma.

    Conclusions:

    • The spatial distribution of CD8 positive cytotoxic T lymphocytes is dysregulated in the prostate TME of cancer patients.
    • Altered cytokine profiles at the mRNA level are associated with prostate cancer.
    • These findings highlight immune cell dysregulation and molecular changes in the prostate cancer microenvironment.