Immunofluorescence localization of nuclear proteins
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Updated: May 28, 2026

Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization
Published on: February 3, 2013
Abstract:
Many nuclear proteins have been successfully localized using immunofluorescence microscopy. These proteins span all nuclear domains, including the nuclear envelope, nuclear lamina, nucleolus, chromatin-associated proteins, and proteins associated with RNA metabolism and nuclear bodies. This article describes a general method for localizing nuclear proteins. Cells grown on coverslips are fixed in either formaldehyde or methanol and permeabilized in Triton X-100. Incubating the cells with primary antibody and fluorescently conjugated secondary antibody allows visualization of the target antigen.
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