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Immunostaining of Whole-Mount Drosophila Testes for 3D Confocal Analysis of Large Spermatocytes
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Immunostaining of Drosophila testes

Silvia Bonaccorsi, Maria G Giansanti, Giovanni Cenci

    Cold Spring Harbor Protocols
    |October 5, 2011
    PubMed

    Fixed Drosophila testis preparations can be immunostained with a variety of antibodies. This article describes a general procedure for immunostaining. The concentration of the primary antibody will vary with both the type of antibody and the type of incubation and should be determined empirically each time.

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