Development and Validation of a Simple Isocratic HPLC Method for Simultaneous Estimation of Phytosterols in Cissus...

Area of Science:

• Phytochemistry
• Analytical Chemistry
• Pharmacognosy

Background:

• Cissus quadrangularis L. is traditionally used in Ayurveda for bone fracture healing.
• Marketed formulations exist, necessitating quality control.
• Phytosterols are key bioactive compounds in C. quadrangularis.

Purpose of the Study:

• To develop and validate a robust RP-HPLC method.
• To quantify phytosterols in C. quadrangularis extracts.
• To ensure reliable quality control of herbal products.

Main Methods:

• Reverse-phase high-performance liquid chromatography (RP-HPLC).
• Cosmosil C(8) column with isocratic elution (acetonitrile:water, 95:5 v/v).
• UV detection at 202 nm.

Main Results:

• Method demonstrated linearity (1-500 μg/ml) with high correlation coefficients.
• Precision, robustness, and ruggedness were within 2% limits.
• High recovery and stability of phytosterols confirmed method accuracy.

Conclusions:

• The developed RP-HPLC method is simple, specific, accurate, and reproducible.
• Suitable for routine quality control analysis of C. quadrangularis phytosterols.
• Supports the quality assurance of C. quadrangularis-based formulations.