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Updated: May 28, 2026
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Preparation Of Neovascular Tissues from Human Glioma Tissues for Quantitative Proteomics Analysis of Tumor Angiogenesis
Published on: March 20, 2026
Correlation between glioblastoma stem-like cells and tumor vascularization
Hu He1, Chao Shi Niu, Ming Wu Li
1Department of Neurosurgery, Anhui Provincial Hospital Affiliated to Anhui Medical University, Hefei, Anhui Province 230001, PR China.
Oncology Reports
|October 6, 2011
Summary
Glioblastoma stem-like cells (GSCs) can differentiate into endothelial cells, directly contributing to tumor blood vessel formation. This discovery offers new therapeutic targets for glioblastoma multiforme (GBM).
Area of Science:
- Neuro-oncology
- Cancer Biology
- Stem Cell Research
Background:
- Glioblastoma multiforme (GBM) is an aggressive brain tumor with poor treatment outcomes.
- Tumor vasculature and glioblastoma stem-like cells (GSCs) are key factors hindering effective GBM treatment.
Purpose of the Study:
- To investigate the role of GSCs in the formation of tumor vasculature in glioblastoma.
- To explore the potential of GSCs to differentiate into endothelial cells.
Main Methods:
- Analysis of 70 glioblastoma samples using immunohistochemistry and double immunofluorescence staining.
- Morphometric analysis of CD133 and Nestin expression in paraffin-embedded sections.
- Evaluation of co-expression of GSC markers (CD133, Nestin) with endothelial markers (CD31) and vascular endothelial growth factor (VEGF).
Main Results:
- CD133 and Nestin were expressed in both tumor and endothelial cells across all GBM samples.
- GSCs were found accumulated around CD31+ blood vessels, with CD133/CD31 or Nestin/CD31 co-expression observed in endothelial cells and GSCs.
- Vascular endothelial growth factor (VEGF) and CD31 were co-expressed in GSCs, indicating their potential for endothelial differentiation.
Conclusions:
- Glioblastoma stem-like cells (GSCs) possess the capacity to differentiate into endothelial cells, directly contributing to tumor vasculogenesis.
- GSCs and tumor vascularization are intrinsically linked in distribution and function.
- These findings reveal a novel mechanism of tumor blood vessel formation and suggest new therapeutic strategies targeting GSCs and tumor vasculature.
