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Mass Spectrometry: Complex Analysis

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

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Updated: May 28, 2026

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)

Published on: May 20, 2013

Separation strategies for untargeted metabolomics.

¹Department of Chemistry, Washington University, School of Medicine, St. Louis, MO 63110, USA. gjpattij@wustl.edu

Journal of Separation Science

|October 6, 2011

Summary

Untargeted metabolomics, crucial for disease research and diagnostics, relies heavily on liquid chromatography (LC) for separating diverse compounds. This review highlights LC

Area of Science:

Biochemistry
Analytical Chemistry
Molecular Biology

Background:

Metabolomics is a key technique for biomarker discovery, molecular diagnostics, and understanding disease.
Global and untargeted approaches are essential for analyzing the metabolome's chemical diversity.
Liquid Chromatography/Mass Spectrometry (LC/MS) is the predominant method for untargeted metabolomics.

Purpose of the Study:

To emphasize the critical role of liquid chromatography (LC) in untargeted metabolomic workflows.
To review LC separation strategies for optimizing untargeted metabolomic analyses.
To provide insights into enhancing metabolite profiling for research and diagnostics.

Main Methods:

Review of existing literature on LC techniques in metabolomics.

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Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis

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Last Updated: May 28, 2026

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)

Published on: May 20, 2013

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis

Published on: July 11, 2014

Analysis of separation strategies for diverse chemical compounds.

Focus on LC's contribution to untargeted metabolomic profiling.

Main Results:

Liquid chromatography is indispensable for separating the complex mixture of metabolites.
Optimization of LC separation is crucial for comprehensive metabolomic profiling.
Effective LC strategies enhance the success of untargeted metabolomic studies.

Conclusions:

Liquid chromatography is a cornerstone of successful untargeted metabolomics.
Strategic LC method development is vital for biomarker discovery and disease research.
Advancements in LC are critical for the future of molecular diagnostics.