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Increased MMP-2 in healthy postmenopausal women
María Luz Muzzio1, Veronica Miksztowicz, Esteban Martín Repetto
1Laboratory of Lipids and Lipoproteins, Physiopathology and Clinical Biochemistry Institute, Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Junin 956 (1113), Buenos Aires, Argentina.
Background:
Matrix metalloproteases 2 (MMP-2) and 9 (MMP-9) are involved in the atherosclerosis process. The objective of the study was to evaluate MMP-2 and MMP-9 activities and other circulating inflammatory factors in healthy postmenopausal women (PMW) as a model of subclinical atherosclerosis.
Methods:
Twenty-three PMW and 13 premenopausal women (PreMW) were selected following established criteria. The main measurements in plasma samples were: lipid-lipoprotein profile, high-sensitivity C-reactive protein (hs-CRP) (immunoturbidimetry), soluble vascular cellular adhesion molecules (sVCAM-1) enzyme-linked immunosorbent assay and MMP activity by zymography.
Results:
The relative areas of MMP-2 were increased in PMW: 1.1 (0.1) versus 0.6 (0.05), P < 0.02. MMP-9 was only detected in three PMW and one PreMW. MMP-2 correlated with HDL-cholesterol (r = -0.51), triglycerides (r = 0.67), apolipoprotein B (r = 0.47), hs-CRP (r = 0.42), homeostasis model assessment (r = 0.53) and waist circumference (r = 0.40), at least P < 0.02. sVCAM-1 showed no difference between groups: 28.7 (5.5) versus 35.5 (20) ng/mL, but correlated with MMP-2 and hs-CRP (r = 0.46 and r = 0.48 respectively, P < 0.05).
Conclusions:
In postmenopause, the increase in MMP-2 reflects the systemic specific inflammatory process that accompanies atherogenesis.
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