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Malaria

Malaria

Malaria pathogenesis in humans reflects a delicate interplay between parasite biology and host response. Clinical illness reflects a host’s immune response to the parasite’s asexual replication cycle, which is often asymptomatic in individuals with partial immunity. From the parasite's perspective, transmission between mosquito and human with minimal host pathology is evolutionarily advantageous. Among the six Plasmodium species infecting humans, P. falciparum and P. vivax dominate in global...

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Related Experiment Video

Updated: May 28, 2026

A Multi-detection Assay for Malaria Transmitting Mosquitoes

A Multi-detection Assay for Malaria Transmitting Mosquitoes

Published on: February 28, 2015

[Nested PCR for malaria detection and Plasmodium species identification].

Yong-xia Shi¹, Ji-cheng Huang, Jin-kun Su

¹Health Quarantine Laboratory, Guangdong Inspection and Quarantine Technology Center, Guangdong Entry-Exit Inspection and Quarantine Bureau, Guangzhou 510700, China. syx0817@yahoo.com.cn

Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi = Chinese Journal of Parasitology & Parasitic Diseases

|October 7, 2011

Summary

A new nested PCR method using small subunit ribosomal RNA (SSU rRNA) gene primers accurately detects Plasmodium species in blood samples. This molecular technique offers improved malaria diagnosis compared to traditional microscopy.

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Detection and Quantification of Plasmodium falciparum in Aqueous Red Blood Cells by Attenuated Total Reflection Infrared Spectroscopy and Multivariate Data Analysis

Detection and Quantification of Plasmodium falciparum in Aqueous Red Blood Cells by Attenuated Total Reflection Infrared Spectroscopy and Multivariate Data Analysis

Published on: November 2, 2018

Area of Science:

Molecular Biology
Parasitology
Medical Diagnostics

Context:

Malaria diagnosis relies on microscopy, which can be limited in sensitivity and species identification.
Accurate and rapid detection of Plasmodium species is crucial for effective treatment and control.

Purpose:

To develop and evaluate a nested PCR method for detecting Plasmodium species using small subunit ribosomal RNA (SSU rRNA) gene sequences.
To compare the diagnostic performance of the nested PCR assay against conventional microscopic examination.

Summary:

Universal and species-specific primers targeting the SSU rRNA gene were designed for Plasmodium detection.
A nested PCR assay was established and applied to 60 blood samples, identifying Plasmodium falciparum, P. vivax, P. ovale, and mixed infections.
The nested PCR showed higher sensitivity than microscopy, with a concordance rate of 76.7% for positive and negative results.

Impact:

The developed nested PCR method provides a sensitive and specific tool for malaria diagnosis and species identification.
Sequence analysis and real-time PCR confirmed discrepant results, validating the accuracy of the nested PCR assay.
This advancement in molecular diagnostics can improve malaria case management and epidemiological surveillance.