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Cell-mediated cytotoxicity against hamster cells transformed by avian sarcoma viruses. 2. Sensitivity of different
Abstract:
The presence of new antigens on different subclones of hamster cells transformed by avian sarcoma viruses was investigated by an in vitro cellular microcytotoxicity assay. The RS 2/3 and RS 2/10 subclones derived from the RS 2 clone, transformed with a Schmidt-Ruppin strain of RSV were highly sensitive to the cytotoxic action of spleen effector cells obtainled from hamsters immunized with RSV-transformed rat cells. The RS 2/6 subclone was sensitive only when 1,000 target cells were seeded and the RS 2/7 subclone was not susceptible to the cytotoxic action of effector cells. The RB 12/1 subclone, derived from the clone RB 12, transformed with a Bryan strain of RSV was poorly sensitive. The comparison between the sensitivity to XC-sensitized spleen cells of the different subclones and their other known properties was discussed.
Insights
Different hamster cell subclones transformed by avian sarcoma viruses show varying sensitivity to immune cell attack. This suggests distinct cell surface antigen expression influencing immune response to RSV-transformed cells.
Area of Science:
- Oncology
- Immunology
- Virology
Background:
- Avian sarcoma viruses induce tumors in various animal models.
- Transformed cells can express new antigens recognized by the immune system.
- Hamster cell lines transformed by Rous Sarcoma Virus (RSV) offer a model to study these antigens.
Purpose of the Study:
- To investigate the presence of new antigens on hamster cell subclones transformed by avian sarcoma viruses.
- To compare the immune cell recognition of different RSV-transformed hamster cell subclones.
Main Methods:
- In vitro cellular microcytotoxicity assay was employed.
- Spleen effector cells from hamsters immunized with RSV-transformed rat cells were used.
- Different RSV-transformed hamster cell subclones (RS 2/3, RS 2/10, RS 2/6, RS 2/7, RB 12/1) were tested for sensitivity.
Main Results:
- RS 2/3 and RS 2/10 subclones were highly sensitive to cytotoxic spleen effector cells.
- RS 2/6 subclone showed sensitivity only at high target cell concentrations (1,000 cells).
- RS 2/7 subclone was not susceptible, and RB 12/1 subclone was poorly sensitive to the cytotoxic action.
Conclusions:
- Hamster cell subclones transformed by RSV exhibit differential expression of cell surface antigens.
- This heterogeneity in antigen expression affects their susceptibility to immune-mediated cytotoxicity.
- The findings contribute to understanding host immune responses against virus-transformed cells.