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Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...

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ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
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Pseudouridines in spliceosomal snRNAs.

Andrew T Yu1, Junhui Ge, Yi-Tao Yu

  • 1Department of Biochemistry and Biophysics, Center for RNA Biology, University of Rochester Medical Center, Rochester, NY 14642, USA.

Protein & Cell
|October 7, 2011
PubMed
Summary

Pseudouridylation of spliceosomal small nuclear RNAs (snRNAs) is crucial for pre-mRNA splicing. These modifications, including stress-induced changes, highlight their regulatory role in gene expression.

Area of Science:

  • Molecular Biology
  • RNA Biology
  • Biochemistry

Background:

  • Spliceosomal small nuclear RNAs (snRNAs) are vital for pre-mRNA splicing.
  • Vertebrate snRNAs undergo extensive post-transcriptional pseudouridylation.
  • Pseudouridines are often located in functionally significant and conserved regions of snRNAs.

Purpose of the Study:

  • To investigate the mechanisms of spliceosomal snRNA pseudouridylation.
  • To explore the functional importance of pseudouridines in snRNAs, particularly U2 snRNA.
  • To examine the potential regulatory role of pseudouridylation under stress conditions.

Main Methods:

  • Analysis of RNA-dependent and RNA-independent pseudouridylation mechanisms.
  • Functional studies on pseudouridines in U2 small nuclear RNA.

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Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection

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Last Updated: May 28, 2026

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
07:31

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection
07:35

Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection

Published on: August 6, 2019

  • Investigation of pseudouridylation patterns under cellular stress.

    • Main Results:

    • Pseudouridylation of spliceosomal snRNAs is catalyzed by distinct RNA-dependent and RNA-independent mechanisms.
    • Nearly all pseudouridines in U2 snRNA are functionally critical for splicing.
    • Pseudouridylation can occur at novel sites under stress, indicating a regulatory function.

    Conclusions:

    • Pseudouridylation is a key modification of spliceosomal snRNAs, essential for splicing efficiency.
    • The functional importance and regulatory potential of snRNA pseudouridylation are significant.
    • Stress-induced pseudouridylation suggests a dynamic role in cellular response and gene regulation.