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MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

MicroRNAs

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Related Experiment Video

Updated: May 28, 2026

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Published on: August 21, 2019

Viruses and microRNAs: a toolbox for systematic analysis.

Diana Lieber¹, Jürgen Haas

¹Max von Pettenkofer Institut, Ludwig-Maximilians-Universität München, München, Germany. lieber@mvp.uni-muenchen.de

Wiley Interdisciplinary Reviews. RNA

|October 7, 2011

Summary

RNA silencing, involving microRNAs (miRNAs), creates complex networks regulating virus-host interactions. High-throughput technologies and bioinformatics are crucial for analyzing these interactions and understanding viral and host mutual regulation.

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MicroRNA-based Regulation of Picornavirus Tropism

MicroRNA-based Regulation of Picornavirus Tropism

Published on: February 6, 2017

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Last Updated: May 28, 2026

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Published on: August 21, 2019

MicroRNA-based Regulation of Picornavirus Tropism

MicroRNA-based Regulation of Picornavirus Tropism

Published on: February 6, 2017

Area of Science:

Virology
Molecular Biology
Genetics

Background:

RNA silencing represents a key regulatory mechanism in virus-host interactions.
Small RNAs, like microRNAs (miRNAs), can target numerous mRNAs, forming intricate regulatory networks.
Understanding these networks is vital for deciphering viral pathogenesis and host defense.

Purpose of the Study:

To review current high-throughput technologies for analyzing miRNA-mediated virus-host interactions.
To summarize findings from high-throughput studies, particularly focusing on herpesviruses.
To highlight the potential of these technologies for broader applications in virology and small RNA research.

Main Methods:

Systematic analysis using high-throughput technologies.
Bioinformatic analysis of miRNA-target interactions.
Review of existing literature and experimental approaches.

Main Results:

High-throughput approaches reveal dense interaction networks between viral and host small RNAs and their targets.
Analysis of herpesvirus interactions demonstrates the complexity of miRNA-mediated regulation.
Current technologies provide powerful tools for dissecting virus-host crosstalk.

Conclusions:

Integrated approaches combining experimental and bioinformatic methods are essential for a comprehensive understanding of virus-host interactions.
The described technologies are broadly applicable to various virus families and other small regulatory RNA pathways.
Further development of these techniques will advance research on viral regulation and host responses.