Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Assembly of Signaling Complexes

Assembly of Signaling Complexes

Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...

Structural Protein Function

Structural Protein Function

Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to form...

Structural Protein Function

Structural Protein Function

Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to form...

Protein Complexes with Interchangeable Parts

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...

Protein Complexes with Interchangeable Parts

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...

Cytoskeletal Accessory Proteins

Cytoskeletal Accessory Proteins

The cytoskeleton is an essential cell component that plays several structural and functional roles. However, the filaments that make up the cytoskeleton cannot function independently and depend on the accessory or ancillary proteins to effectively carry out their function. Accessory proteins associate with cytoskeletal filaments and their monomers, aiding filament formation and function. They also help in the cross-communication among cytoskeletal filaments. Cytoskeletal accessory proteins are...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A class of deep intronic <i>IGHMBP2</i> variants activate a shared cryptic splice donor, enabling correction of select variants with a single antisense oligonucleotide.

medRxiv : the preprint server for health sciences·2026

Same author

Ampyrone is a direct agonist of human tyrosinase and a potential therapeutic for hypopigmentation disorders.

JCI insight·2026

Same author

Targeting melanosome pH is an effective method for the treatment of oculocutaneous albinism.

bioRxiv : the preprint server for biology·2026

Same author

Haplotype-Based Analysis of OCA2 Variants in Oculocutaneous Albinism.

Pigment cell & melanoma research·2026

Same author

Translating multi-omics into healthcare: requisites for scalable and equitable implementation.

Human genomics·2026

Same author

Clinical and Genetic Characteristics of Free Sialic Acid Storage Disorder.

Journal of inherited metabolic disease·2026

Same journal

Integration of breaking-point transition probabilities and specific open chromatin region features for early cancer detection.

Cell communication and signaling : CCS·2026

Same journal

A pan-cancer single-cell atlas uncovers the role of sex hormones and chromosomes in sex-divergent reprogramming of the tumor microenvironment.

Cell communication and signaling : CCS·2026

Same journal

HIV Tat-activated microglial extracellular vesicles induce neuronal iron dysregulation and synaptodendritic injury.

Cell communication and signaling : CCS·2026

Same journal

Targeting AIF to trigger RIPKs/MLKL necroptosis: a disulfiram-based strategy to reverse paclitaxel resistance in ovarian cancer.

Cell communication and signaling : CCS·2026

Same journal

Compositional recalibrations of cardiolipin integrate loss of stearoyl-CoA desaturase 1 activity with mitochondrial decay in lipid-laden pancreatic β-cells.

Cell communication and signaling : CCS·2026

Same journal

A bispecific-aptamer TSHR-CD80-1 treats thyroid eye disease by inhibiting CD8<sup>+</sup> T cell-fibroblast interactions.

Cell communication and signaling : CCS·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 28, 2026

Pulldown Assay Coupled with Co-Expression in Bacteria Cells as a Time-Efficient Tool for Testing Challenging Protein-Protein Interactions

Pulldown Assay Coupled with Co-Expression in Bacteria Cells as a Time-Efficient Tool for Testing Challenging Protein-Protein Interactions

Published on: December 23, 2022

RACK1, A multifaceted scaffolding protein: Structure and function.

David R Adams¹, Dorit Ron, Patrick A Kiely

¹Department of Life Sciences, and Materials and Surface Science Institute, University of Limerick, Limerick, Ireland. Patrick.Kiely@ul.ie.

Cell Communication and Signaling : CCS

|October 8, 2011

Summary

This summary is machine-generated.

Receptor for Activated C Kinase 1 (RACK1) is a versatile protein involved in cell signaling and function. This review explores RACK1

More Related Videos

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

Published on: January 11, 2017

Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae

Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae

Published on: January 10, 2018

Related Experiment Videos

Last Updated: May 28, 2026

Pulldown Assay Coupled with Co-Expression in Bacteria Cells as a Time-Efficient Tool for Testing Challenging Protein-Protein Interactions

Pulldown Assay Coupled with Co-Expression in Bacteria Cells as a Time-Efficient Tool for Testing Challenging Protein-Protein Interactions

Published on: December 23, 2022

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

Published on: January 11, 2017

Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae

Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae

Published on: January 10, 2018

Area of Science:

Molecular Biology
Cell Biology
Biochemistry

Background:

Receptor for Activated C Kinase 1 (RACK1) is a WD-repeat protein structurally similar to G protein β subunits.
RACK1 features a seven-bladed β-propeller structure crucial for protein interactions.
It acts as a scaffold, mediating protein transport, localization, and stability.

Purpose of the Study:

To review the gene and structure of RACK1.
To elucidate RACK1's role in cellular signaling pathways.
To discuss how posttranslational modifications influence RACK1 localization and function.

Main Methods:

Literature review of recent studies on RACK1.
Analysis of RACK1's interactions with cellular components (ribosomes, receptors, nuclear proteins).
Examination of RACK1's involvement in physiological and pathological processes.

Main Results:

RACK1 mediates diverse cellular functions by interacting with various protein complexes.
Posttranslational modifications are key regulators of RACK1's subcellular distribution.
RACK1 is implicated in development, cell migration, CNS function, and circadian rhythms.

Conclusions:

RACK1 is a critical mediator in numerous cellular pathways.
Understanding RACK1's role in disease is an emerging area of research.
RACK1's structural and functional versatility makes it a significant focus in cell biology.