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Related Concept Videos

Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012 for this...
iPS Cell Differentiation01:22

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.

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Related Experiment Video

Updated: May 28, 2026

The MultiBac Protein Complex Production Platform at the EMBL
13:51

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Published on: July 11, 2013

Generation of iPS cells using a BacMam multigene expression system.

Yoko Takata1, Hiroe Kishine, Takefumi Sone

  • 1Department of Molecular Biology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan.

Cell Structure and Function
|October 8, 2011
PubMed
Summary

Researchers generated induced pluripotent stem cells (iPS cells) from mouse embryonic fibroblasts (MEF) using a novel BacMam system. This method efficiently delivers reprogramming transcription factors for rapid iPS cell generation.

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Area of Science:

  • Stem Cell Biology
  • Molecular Biology
  • Gene Delivery Systems

Background:

  • Induced pluripotent stem cells (iPSCs) hold promise for regenerative medicine.
  • Efficient and reliable methods for iPSC generation are crucial for research and therapeutic applications.
  • Traditional reprogramming methods can be time-consuming and have variable efficiencies.

Purpose of the Study:

  • To develop an efficient BacMam-based system for generating iPS cells from mouse embryonic fibroblasts (MEFs).
  • To optimize the delivery of reprogramming factors using a polycistronic expression vector.
  • To evaluate the efficiency and timeline of iPSC generation using this novel system.

Main Methods:

  • Utilized a BacMam transduction system with a polycistronic plasmid expressing Oct4, Klf4, Sox2, and c-Myc (OKSM).
  • Cloned OKSM genes into a single open reading frame (ORF) using self-cleaving 2A peptides under the CAG promoter.
  • Transduced primary MEF cells with BacMam particles carrying the OKSM cassette, assessing transduction efficiency with a Venus reporter gene.

Main Results:

  • Achieved high transduction efficiency (64-98%) of MEF cells with BacMam particles.
  • Generated iPS cell colonies within 15-24 days after three successive transductions or even a single transduction.
  • Confirmed successful reprogramming by positive staining for Nanog, SSEA-1, and alkaline phosphatase in derived iPS cell lines.

Conclusions:

  • The BacMam transduction system provides an efficient and rapid method for generating iPS cells from MEFs.
  • A single transduction can be sufficient for generating fully reprogrammed iPS cell lines.
  • This system offers an advantageous approach for iPSC generation and research.